Supplementary Materials

Supplementary Materials for:

Hedgehog induces formation of PKA-Smoothened complexes to promote Smoothened phosphorylation and pathway activation

Shuang Li, Guoqiang Ma, Bing Wang, Jin Jiang*

*Corresponding author. E-mail: jin.jiang@utsouthwestern.edu

This PDF file includes:

  • Fig. S1. Hh signaling stabilizes PKAc depending on Smo C-tail.
  • Fig. S2. Characterization of AKAR3 and Myr-AKAR3 in S2 cells.
  • Fig. S3. Hh increases Myr-AKAR3 FRET in S2 cells.
  • Fig. S4. Hh signaling increases Myr-AKAR3 FRET in wing discs.
  • Fig. S5. Characterization of PKAc-Smo interaction by coimmunoprecipitation assay.
  • Fig. S6. Multiple basic clusters mediate binding of PKAc to the SAID domain.
  • Fig. S7. Hh induces colocalization between mC* and truncated Smo independent of endogenous Smo.
  • Fig. S8. Hh induces FRET between CFP-tagged Smo variants and mC*-YFP.
  • Fig. S9. Characterization of Smo expression driven by different Gal4 drivers.
  • Fig. S10. Smo-PKAc complex formation stabilizes PKAc.
  • Fig. S11. Hh switches the binding of PKAc from Cos2 to Smo.

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Citation: S. Li, G. Ma, B. Wang, J. Jiang, Hedgehog induces formation of PKA-Smoothened complexes to promote Smoothened phosphorylation and pathway activation. Sci. Signal. 7, ra62 (2014).

© 2014 American Association for the Advancement of Science