Supplementary Materials

Supplementary Materials for:

Phosphorylation of the vacuolar anion exchanger AtCLCa is required for the stomatal response to abscisic acid

Stefanie Wege, Alexis De Angeli, Marie-Jo Droillard, Laetitia Kroniewicz, Sylvain Merlot, David Cornu, Franco Gambale, Enrico Martinoia, Hélène Barbier-Brygoo, Sébastien Thomine, Nathalie Leonhardt, Sophie Filleur*

*Corresponding author. E-mail: sophie.filleur@isv.cnrs-gif.fr

This PDF file includes:

  • Fig. S1. The ABA response of stomata aperture is similar in the presence of nitrate instead of chloride.
  • Fig. S2. Purity and activity of recombinant OST1 and OST1-G33R.
  • Fig. S3. Characterization of AtCLCa1–86 phosphorylation by mass spectrophotometry.
  • Fig. S4. Activity of recombinant OST1 in patch-clamp buffer and effect of OST1 on current families evoked in clca-2 knockout mutant.
  • Fig. S5. Abundance of AtCLCa mRNA in the different genotypes used in the study.

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Citation: S. Wege, A. De Angeli, M.-J. Droillard, L. Kroniewicz, S. Merlot, D. Cornu, F. Gambale, E. Martinoia, H. Barbier-Brygoo, S. Thomine, N. Leonhardt, S. Filleur, Phosphorylation of the vacuolar anion exchanger AtCLCa is required for the stomatal response to abscisic acid. Sci. Signal. 7, ra65 (2014).

© 2014 American Association for the Advancement of Science