Supplementary Materials

Supplementary Materials for:

AKAP150-dependent cooperative TRPV4 channel gating is central to endothelium-dependent vasodilation and is disrupted in hypertension

Swapnil K. Sonkusare, Thomas Dalsgaard, Adrian D. Bonev, David C. Hill-Eubanks, Michael I. Kotlikoff, John D. Scott, Luis F. Santana, Mark T. Nelson*

*Corresponding author. E-mail: mark.nelson@uvm.edu

This PDF file includes:

  • Fig. S1. Relationship between GSK101 concentration and TRPV4-mediated whole cell K+ currents.
  • Fig. S2. TRPV4 Ca2+ sparklets at MEPs recorded in the presence of GSK101 and CPA.
  • Fig. S3. CCh activates TRPV4 sparklets at MEPs through the PLC-DAG-PKC pathway in the presence of CPA and GSK101.
  • Fig. S4. Bradykinin activates TRPV4 sparklets at MEPs in a PKC-dependent manner.
  • Fig. S5. PKCα is not preferentially localized at MEPs.
  • Fig. S6. Sparklet sites have a maximum of four TRPV4 channels.
  • Fig. S7. Ratio of the experimentally observed PO of TRPV4 channels to the PO predicted by the binomial distribution assuming independent gating of TRPV4 channels.
  • Fig. S8. Inhibition or activation of the GqPCR-PKC-TRPV4 pathway does not alter the functional coupling of TRPV4 channels.
  • Fig. S9. Inhibition of PKC or IK channels attenuates dilation to CCh.
  • Fig. S10. The EDH component of CCh-induced vasodilation is abolished in Ang II–induced hypertension.
  • Fig. S11. CCh-induced increases in whole-cell Ca2+ in ECs and IK channel localization at MEPs are unaltered in hypertension.
  • Table S1. Number and density of IK, SK, and TRPV4 channels in ECs.
  • Table S2. Internal diameters of CCh-dilated third-order mesenteric arteries from NT and hypertensive mice.
  • Table S3. Internal diameters of GSK101-dilated third-order mesenteric arteries from NT and hypertensive mice.
  • Table S4. General properties of mesenteric arteries and ECs from NT and hypertensive (HT) mice.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.wmv format). Z-stack of AKAP150 staining images across the z axis from the luminal surface to the SMC layer in NT mice.
  • Movie S2 (.wmv format). Z-stack of AKAP150 staining images across the z axis from the luminal surface to the SMC layer in hypertensive mice.

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Citation: S. K. Sonkusare, T. Dalsgaard, A. D. Bonev, D. C. Hill-Eubanks, M. I. Kotlikoff, J. D. Scott, L. F. Santana, M. T. Nelson, AKAP150-dependent cooperative TRPV4 channel gating is central to endothelium-dependent vasodilation and is disrupted in hypertension. Sci. Signal. 7, ra66 (2014).

© 2014 American Association for the Advancement of Science