Supplementary Materials

Supplementary Materials for:

Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation

Hiroki Onuma, Toru Komatsu,* Makoto Arita, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, Takanari Inoue*

*Corresponding author. E-mail: tkomatsu@mol.f.u-tokyo.ac.jp (T.K.); jctinoue@jhmi.edu (T.I.)

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  • Fig. S1. Analysis of various constructs for rapamycin-dependent dimerization.
  • Fig. S2. Rapamycin has no side effects on protein localization.
  • Fig. S3. Analysis of the dynamics and kinetics of the DISplay procedure.
  • Fig. S4. The DISplay can be used with different cell types.
  • Fig. S5. Gibberellin-based dimerization system.
  • Fig. S6. Analysis of the localization of Golgi-anchored proteins and the effect of rapamycin on organelles.
  • Fig. S7. Analysis of intracellular vesicles formed de novo after dimerization of FYG-Myc and RC-CAAX.
  • Fig. S8. Colocalization of FY-giantin and VSVG protein.
  • Fig. S9. Localization of Golgi-anchored protein in HeLa cells treated with BFA.
  • Fig. S10. Localization of Golgi-anchored protein in HeLa cells treated with monensin.
  • Fig. S11. Localization of Golgi-anchored protein in HeLa cells at 4°C.
  • Fig. S12. Dimerization of Golgi-anchored protein constructs.
  • Fig. S13. Labeling at the cell surface with the SNAP tag.
  • Fig. S14. Localization of C2-fused protein.
  • Fig. S15. Immunostaining of the Myc tag in cells expressing FYG-Myc-C2.
  • Fig. S16. Flow cytometric analysis of apoptotic Jurkat cells.
  • Fig. S17. Fluorescence micrographs of HeLa cells bound to apoptotic Jurkat cells.
  • Fig. S18. Attachment of apoptotic Jurkat cells to the surface of RAW264.7 cells.
  • Fig. S19. Actin structures in cells expressing Rac1 (CA).
  • Fig. S20. Fluorescence micrographs of COS-7 cells engulfing Jurkat cells.
  • Table S1. Statistical OR analysis of engulfment assays with HeLa cells.
  • Table S2. Statistical RR analysis of engulfment assays with COS-7 cells.
  • Table S3. Statistical OR analysis of engulfment assays with COS-7 cells.
  • Legends for movies S1 to S3
  • Reference (35)

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Citation: H. Onuma, T. Komatsu, M. Arita, K. Hanaoka, T. Ueno, T. Terai, T. Nagano, T. Inoue, Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation. Sci. Signal. 7, rs4 (2014).

© 2014 American Association for the Advancement of Science