Supplementary Materials

Supplementary Materials for:

Rab13 acts downstream of the kinase Mst1 to deliver the integrin LFA- 1 to the cell surface for lymphocyte trafficking

Akihiko Nishikimi, Sayaka Ishihara, Madoka Ozawa, Kan Etoh, Mitsunori Fukuda, Tatsuo Kinashi, Koko Katagiri*

*Corresponding author. E-mail: katagirk@kitasato-u.ac.jp

This PDF file includes:

  • Fig. S1. Effects of Rab13 on the kinase activity of Mst1 and the localization of Rab13 and LFA-1.
  • Fig. S2. Involvement of vesicular transport in the clustering of LFA-1.
  • Fig. S3. Analysis of the motility of Rab13-knockdown cells.
  • Fig. S4. Quantitation of the accumulation of Rab13 at contact sites between T cells and APCs.
  • Fig. S5. LFA-1 associates with Mst1 and is cotranslocated to the leading edge with Rab13.
  • Fig. S6. Involvement of VASP in the clustering of LFA-1 in response to CXCL12.
  • Fig. S7. Colocalization of Rab13 and myosin Va.
  • Fig. S8. Generation of Rab13-deficient mice and analysis of the cell surface markers and chemotaxis of Rab13-deficient T cells.
  • Fig. S9. Model for the Rab13- and Mst1-dependent activation of LFA-1 in lymphocytes.
  • Table S1. Summary of the binding of Rab family GTPases to Mst1.
  • Legends for movies S1 to S14.

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.63 MB

Other Supplementary Material for this manuscript includes the following:

  • Movies S1 to S14 (.mov format).

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Citation: A. Nishikimi, S. Ishihara, M. Ozawa, K. Etoh, M. Fukuda, T. Kinashi, K. Katagiri, Rab13 acts downstream of the kinase Mst1 to deliver the integrin LFA-1 to the cell surface for lymphocyte trafficking. Sci. Signal. 7, ra72 (2014).

© 2014 American Association for the Advancement of Science