Supplementary Materials

Supplementary Materials for:

The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility

Tai Kiuchi, Elena Ortiz-Zapater, James Monypenny, Daniel R. Matthews, Lan K. Nguyen, Jody Barbeau, Oana Coban, Katherine Lawler, Brian Burford, Daniel J. Rolfe, Emanuele de Rinaldis, Dimitra Dafou, Michael A. Simpson, Natalie Woodman, Sarah Pinder, Cheryl E. Gillett, Viviane Devauges, Simon P. Poland, Gilbert Fruhwirth, Pierfrancesco Marra, Ykelien L. Boersma, Andreas Plückthun, William J. Gullick, Yosef Yarden, George Santis, Martyn Winn, Boris N. Kholodenko, Marisa L. Martin-Fernandez, Peter Parker, Andrew Tutt, Simon M. Ameer-Beg,* Tony Ng*

*Corresponding author. E-mail: simon.ameer-beg@kcl.ac.uk (S.M.A.-B.); tony.ng@kcl.ac.uk (T.N.)

This PDF file includes:

  • Text S1. Development of a core model of the EGFR–ErbB4 CYT2 interaction network.
  • Fig. S1. Evaluation of the subcellular localization of tagged ErbB4 isoforms by immunofluorescence.
  • Fig. S2. Abundance of ErbB4 exon–specific sequences in 404 human breast cancer samples within the TCGA RNASeq database.
  • Fig. S3. The effect of knockdown of individual ErbB family members on EGF-dependent EGFR degradation.
  • Fig. S4. Schematic of conserved and putative c-Cbl and Grb2 binding sites in ErbB4 JMa CYT1.
  • Fig. S5. Sequence homology alignment of the EGFR and ErbB4 CYT2 dimerization domains and associated residues targeted for mutagenesis.
  • Fig. S6. Kinetic model of the core EGFR:ErbB4 CYT2 interaction network.
  • Fig. S7. Simulated steady-state concentrations of total EGFR over random parameter sets.
  • Table S1. Reactions and reaction rates of the core EGFR–ErbB4 CYT2 interaction model.
  • Table S2. Ordinary differential equations of the core EGFR–ErbB4 CYT2 interaction model.
  • Legends for movies S1 to S4
  • References (8588)

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 989 KB

Other Supplementary Material for this manuscript includes the following: updated 9 September 2014

  • Movie S1 (.avi format). Migration of EGFR- and EGFP-expressing MCF-7 cells in an HB-EGF gradient.
  • Movie S2 (.avi format). Migration of ErbB4 CYT2 mICD–EGFP–expressing MCF-7 cells in an HB-EGF gradient.
  • Movies S3 and S4 (.avi format). Two examples of EGFR- and ErbB4 CYT2–expressing MCF-7 cells chemotaxing toward an HB-EGF gradient.

[Download Movie S1]

[Download Movie S2]

[Download Movie S3]

[Download Movie S4]


Citation: T. Kiuchi, E. Ortiz-Zapater, J. Monypenny, D. R. Matthews, L. K. Nguyen, J. Barbeau, O. Coban, K. Lawler, B. Burford, D. J. Rolfe, E. de Rinaldis, D. Dafou, M. A. Simpson, N. Woodman, S. Pinder, C. E. Gillett, V. Devauges, S. P. Poland, G. Fruhwirth, P. Marra, Y. L. Boersma, A. Plückthun, W. J. Gullick, Y. Yarden, G. Santis, M. Winn, B. N. Kholodenko, M. L. Martin-Fernandez, P. Parker, A. Tutt, S. M. Ameer-Beg, T. Ng, The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility. Sci. Signal. 7, ra78 (2014).

© 2014 American Association for the Advancement of Science