Supplementary Materials

Supplementary Materials for:

Diacylglycerol kinase α establishes T cell polarity by shaping diacylglycerol accumulation at the immunological synapse

Anne Chauveau, Audrey Le Floc'h, Niels S. Bantilan, Gary A. Koretzky, Morgan Huse*

*Corresponding author. E-mail: husem@mskcc.org

This PDF file includes:

  • Fig. S1. Photoactivatable ligands for the 5C.C7 and OT-1 TCRs.
  • Fig. S2. DGK-α is required for MTOC polarization in TCR photoactivation experiments.
  • Fig. S3. Localization patterns of DGK-α variants at the immunological synapse.
  • Fig. S4. PI3K activity promotes MTOC polarization and synaptic DAG.
  • Legends for movies S1 to S5

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Technical Details

Format: Adobe Acrobat PDF

Size: 669 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Localized TCR photoactivation induces MTOC reorientation.
  • Movie S2 (.mov format). DGK-ζ is dispensable for MTOC reorientation.
  • Movie S3 (.mov format). DGK-α is required for MTOC reorientation.
  • Movie S4 (.mov format). Localized TCR photoactivation stimulates focal DAG accumulation.
  • Movie S5 (.mov format). DGK-α is required for proper DAG gradient formation.

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Citation: A. Chauveau, A. Le Floc'h, N. S. Bantilan, G. A. Koretzky, M. Huse, Diacylglycerol kinase α establishes T cell polarity by shaping diacylglycerol accumulation at the immunological synapse. Sci. Signal. 7, ra82 (2014).

© 2014 American Association for the Advancement of Science