Supplementary Materials for:

T cell receptor stimulation impairs IL-7 receptor signaling by inducing expression of the microRNA miR-17 to target Janus kinase 1

Gil Katz, Leonid A. Pobezinsky, Susanna Jeurling, Miho Shinzawa, Francois Van Laethem, Alfred Singer

*Corresponding author. E-mail: singera@mail.nih.gov

This PDF file includes:

• Fig. S1. Analysis of total protein synthesis in unstimulated and TCR-stimulated cells.
• Fig. S2. Imperfect match for microRNAs that potentially bind to the 3′UTR of Jak1 mRNA.
• Fig. S3. Effect of TCR signaling on the expression of microRNAs in the miR-17~92 cluster.
• Fig. S4. Analysis of GFP abundance in Jurkat cells stably transfected with plasmids encoding GFP and either scrambled control microRNA or miR-17.
• Fig. S5. IL-7 signaling is impaired in Ctla4^−/− mice.
• Table S1. Primers used for real-time PCR analysis.

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Citation: G. Katz, L. A. Pobezinsky, S. Jeurling, M. Shinzawa, F. Van Laethem, A. Singer, T cell receptor stimulation impairs IL-7 receptor signaling by inducing expression of the microRNA miR-17 to target Janus kinase 1. Sci. Signal. 7, ra83 (2014).

© 2014 American Association for the Advancement of Science