Supplementary Materials

Supplementary Materials for:

Protective effect of the long pentraxin PTX3 against histone-mediated endothelial cell cytotoxicity in sepsis

Kenji Daigo, Makoto Nakakido, Riuko Ohashi, Rie Fukuda, Koichi Matsubara, Takashi Minami, Naotaka Yamaguchi, Kenji Inoue, Shuying Jiang, Makoto Naito, Kouhei Tsumoto, Takao Hamakubo*

*Corresponding author. E-mail: hamakubo{at}qbm.rcast.u-tokyo.ac.jp

This PDF file includes:

  • Fig. S1. Analysis of histone-PTX3 interactions by ELISA.
  • Fig. S2. Analysis of histone-PTX3 interactions by SPR.
  • Fig. S3. Analysis of the interaction between histone peptides and PTX3.
  • Fig. S4. UV-visible spectrum of a histone-PTX3 mixture.
  • Fig. S5. Assessments of histone-PTX3 aggregation.
  • Fig. S6. PTX3-mediated protection against cytotoxicity that is caused by the association of histones with the endothelium.
  • Fig. S7. Effects of PTX3 in histone-infused mice.
  • Fig. S8. Time-course analysis of plasma histone H3 concentrations in LPS-treated mice.
  • Table S1. List of the histone H3 and H4 fragment peptides.

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Citation: K. Daigo, M. Nakakido, R. Ohashi, R. Fukuda, K. Matsubara, T. Minami, N. Yamaguchi, K. Inoue, S. Jiang, M. Naito, K. Tsumoto, T. Hamakubo, Protective effect of the long pentraxin PTX3 against histone-mediated endothelial cell cytotoxicity in sepsis. Sci. Signal. 7, ra88 (2014).

© 2014 American Association for the Advancement of Science