Supplementary Materials

Supplementary Materials for:

TGF-β–induced epithelial-to-mesenchymal transition proceeds through stepwise activation of multiple feedback loops

Jingyu Zhang, Xiao-Jun Tian, Hang Zhang, Yue Teng, Ruoyan Li, Fan Bai, Subbiah Elankumaran,* Jianhua Xing*

*Corresponding author. E-mail: jxing{at}vt.edu (J.X.); kumarans{at}vt.edu (S.E.)

This PDF file includes:

  • Text S1. General model for the miRNA-mediated regulation of mRNA.
  • Text S2. The revised CBS model for EMT system.
  • Text S3. Qualitatively, but not quantitatively, different predictions can distinguish the CBS and TBS models for EMT.
  • Fig. S1. Flow cytometric analysis of TGF-β1–induced cells recultured in the absence of TGF-β1 confirms that the SNAIL1/miR-34 module functions as a bistable switch.
  • Fig. S2. Temporal dynamics of TGF-β1–induced EMT in MCF10A cells.
  • Fig. S3. Immunofluorescence of E-cadherin and vimentin at different time points in response to 4 ng/ml TGF-β1.
  • Fig. S4. Analyses of the three subgroups at the indicated time points by flow cytometry in response to different concentrations of TGF-β1.
  • Fig. S5. Generic model for the regulation of mRNA by miRNA.
  • Fig. S6. Full reaction diagram of miRNA regulation of mRNA with four binding sites when considering all the possible miRNA-mRNA complexes.
  • Table S1. Comparison between the original CBS and TCS models.
  • Table S2. Primers used for quantitative RT-PCR.
  • Table S3. Parameters used in the present model studies.
  • Table S4. Initial conditions of the variables in the model.
  • Legend for model S1
  • References (6771)

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

  • Model S1. Computer code of the theoretical model.

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Citation: J. Zhang, X.-J. Tian, H. Zhang, Y. Teng, R. Li, F. Bai, S. Elankumaran, J. Xing, TGF-β–induced epithelial-to-mesenchymal transition proceeds through stepwise activation of multiple feedback loops. Sci. Signal. 7, ra91 (2014).

© 2014 American Association for the Advancement of Science