Supplementary Materials

Supplementary Materials for:

Partial inhibition of gp130-Jak-Stat3 signaling prevents Wnt–β-catenin–mediated intestinal tumor growth and regeneration

Toby J. Phesse, Michael Buchert, Emma Stuart, Dustin J. Flanagan, Maree Faux, Shoukat Afshar-Sterle, Francesca Walker, Hui-Hua Zhang, Cameron J. Nowell, Robert Jorissen, Chin Wee Tan, Yumiko Hirokawa, Moritz F. Eissmann, Ashleigh R. Poh, Jordane Malaterre, Helen B. Pearson, David G. Kirsch, Paolo Provero, Valeria Poli, Robert G. Ramsay, Oliver Sieber, Antony W. Burgess, Dennis Huszar, Elizabeth Vincan, Matthias Ernst*

*Corresponding author. E-mail: matthias.ernst{at}wehi.edu.au

This PDF file includes:

  • Fig. S1. gp130-Jak-Stat3 signaling regulates intestinal regeneration and tumorigenesis.
  • Fig. S2. gp130-Jak-Stat3 signaling limits induced colonic tumorigenesis in Lgr5CreERT2; Apcfl/fl mice and human CRC xenografts.
  • Fig. S3. Gene signature for Wnt and gp130-Jak-Stat3 signaling coexists in human CRCs.
  • Fig. S4. Xenograft growth is suppressed by partial inhibition of the gp130-Jak-Stat3 pathway without affecting Stat1.
  • Fig. S5. Stimulation of gp130-Jak-Stat3 signaling does not affect aberrant Wnt–β-catenin signaling in the SW480 human CRC cell line.
  • Fig. S6. Inhibition of gp130-Jak-Stat3 signaling does not affect intestinal homeostasis.
  • Fig. S7. Inhibition of gp130-Jak-Stat3 signaling relieves Bmi-1–mediated repression of the gene encoding p21 in intestinal tumors.
  • Table S1. Primer sequences used for RT-qPCR.

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Citation: T. J. Phesse, M. Buchert, E. Stuart, D. J. Flanagan, M. Faux, S. Afshar-Sterle, F. Walker, H.-H. Zhang, C. J. Nowell, R. Jorissen, C. W. Tan, Y. Hirokawa, M. F. Eissmann, A. R. Poh, J. Malaterre, H. B. Pearson, D. G. Kirsch, P. Provero, V. Poli, R. G. Ramsay, O. Sieber, A. W. Burgess, D. Huszar, E. Vincan, M. Ernst, Partial inhibition of gp130-Jak-Stat3 signaling prevents Wnt–β-catenin–mediated intestinal tumor growth and regeneration. Sci. Signal. 7, ra92 (2014).

© 2014 American Association for the Advancement of Science