Supplementary Materials

Supplementary Materials for:

Temporal and spatial regulation of epsin abundance and VEGFR3 signaling are required for lymphatic valve formation and function

Xiaolei Liu, Satish Pasula, Hoogeun Song, Kandice L. Tessneer, Yunzhou Dong, Scott Hahn, Tadayuki Yago, Megan L. Brophy, Baojun Chang, Xiaofeng Cai, Hao Wu, John McManus, Hirotake Ichise, Constantin Georgescu, Jonathan D. Wren, Courtney Griffin, Lijun Xia, R. Sathish Srinivasan, Hong Chen*

*Corresponding author. E-mail: hong-chen{at}omrf.org

This PDF file includes:

  • Fig. S1. Generation of constitutive and tamoxifen-inducible LEC-DKO or LEC-iDKO mice.
  • Fig. S2. Normal collecting lymphatic vessel development before lymphatic valve formation in LEC-DKO embryos.
  • Fig. S3. Lymphatic capillary development in LEC-DKO embryos at E16.5 and E18.5.
  • Fig. S4. Dilated lymphatic capillary development in epsin-deficient mice at P0.
  • Fig. S5. Abnormal lymphatic valve development in LEC-iDKO mice.
  • Fig. S6. Normal lymphovenous valve development in LEC-DKO embryos.
  • Fig. S7. Epsin abundance in valve-covering and lymphatic trunk regions.
  • Fig. S8. Increased epsin abundance under laminar shear stress.
  • Fig. S9. VEGFR3 mRNA expression in mesenteric LECs during early developmental stages.
  • Fig. S10. Epsin loss did not affect VEGFR3 mRNA expression.
  • Fig. S11. Increased lymphatic sprouting and proliferation in adult LEC-DKO mice with administration of VEGF-C.
  • Fig. S12. Improved lymphatic function by reducing VEGFR3 abundance or activity in epsin-deficient mice.
  • Fig. S13. Improved lymphatic capillary development by reducing VEGFR3 abundance or activity in epsin-deficient mice.
  • Fig. S14. Schematic model of how flow-mediated differential induction of epsin spatially and temporally regulates VEGFR3 abundance and lymphatic valve formation.

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Citation: X. Liu, S. Pasula, H. Song, K. L. Tessneer, Y. Dong, S. Hahn, T. Yago, M. L. Brophy, B. Chang, X. Cai, H. Wu, J. McManus, H. Ichise, C. Georgescu, J. D. Wren, C. Griffin, L. Xia, R. S. Srinivasan, H. Chen, Temporal and spatial regulation of epsin abundance and VEGFR3 signaling are required for lymphatic valve formation and function. Sci. Signal. 7, ra97 (2014).

© 2014 American Association for the Advancement of Science