Supplementary materials

Supplementary Materials for:

Reciprocal allosteric regulation of p38γ and PTPN3 involves a PDZ domain–modulated complex formation

Kai-En Chen, Shu-Yu Lin, Mei-Ju Wu, Meng-Ru Ho, Abirami Santhanam, Chia-Cheng Chou, Tzu-Ching Meng,* Andrew H.-J. Wang*

*Corresponding author. E-mail: tcmeng{at}gate.sinica.edu.tw (T.-C.M.); ahjwang{at}gate.sinica.edu.tw (A.H.-J.W.)

This PDF file includes:

  • Fig. S1. MS spectra of p38γ phosphorylation.
  • Fig. S2. Activation of p38γ is required for the interaction with N3PTPD811A/C842S.
  • Fig. S3. Superimposition of the wild-type N3PTP and phosphopeptide complex structures.
  • Fig. S4. Modeling of the phospho-p38γ activation loop from hidden to extended conformation.
  • Fig. S5. MS/MS spectra of intramolecular and intermolecular cross-linked peptides correspond to the linkage of N3PDZ-PTPD811A/C842S and phospho-p38γ.
  • Fig. S6. Homology model of N3PDZ.
  • Fig. S7. Cross-linking and MS derived N3PDZ-PTP–p-p38γ complex using the best-fit model 2 of SAXS as template.
  • Fig. S8. Representative HADDOCK structures of N3PDZ-PTPD811A/C842S from each cluster.
  • Fig. S9. E-loop sequence alignment between FERM- and KIM-containing PTPs.
  • Table S1. Summary of protein constructs used in this study.
  • Table S2. SAXS data collection and analysis details.
  • Table S3. Comparison of SAXS-derived envelopes between different PTP-MAPK complexes.
  • Table S4. MS identification of cross-linked peptides between N3PDZ-PTPD811A/C842S and phospho-p38γ using BS3 cross-linker.
  • Table S5. MS identification of cross-linked peptides between N3PTPD811A/C842S and phospho-p38γ using BS3 cross-linker.
  • Table S6. MS identification of cross-linked peptides of N3PDZ-PTPD811A/C842S using BS3 cross-linker.
  • References (6670)

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Citation: K.-E. Chen, S.-Y. Lin, M.-J. Wu, M.-R. Ho, A. Santhanam, C.-C. Chou, T.-C. Meng, A. H.-J. Wang, Reciprocal allosteric regulation of p38γ and PTPN3 involves a PDZ domain–modulated complex formation. Sci. Signal. 7, ra98 (2014).

© 2014 American Association for the Advancement of Science