Supplementary Materials

Supplementary Materials for:

Resistance to dual blockade of the kinases PI3K and mTOR in KRAS-mutant colorectal cancer models results in combined sensitivity to inhibition of the receptor tyrosine kinase EGFR

Peter J. Belmont,* Ping Jiang, Trevor D. McKee, Tao Xie, Jason Isaacson, Nicole E. Baryla, Jatin Roper, Mark J. Sinnamon, Nathan V. Lee, Julie L. C. Kan, Oivin Guicherit, Bradly G. Wouters, Catherine A. O'Brien, David Shields, Peter Olson, Todd VanArsdale, Scott L. Weinrich, Paul Rejto, James G. Christensen, Valeria R. Fantin, Kenneth E. Hung, Eric S. Martin*

*Corresponding author. E-mail: esmartin.phd{at}gmail.com (E.S.M.); petebelmont{at}gmail.com (P.J.B.)

This PDF file includes:

  • Fig. S1. siFOXO3a knockdown in parental and 502R cells.
  • Fig. S2. Chemical screening to identify combinations that yield sensitivity to PF-04691502 in resistant cells.
  • Fig. S3. Schematic of RNA-Seq profiling.
  • Fig. S4. Characterization of resistance to PF-04691502 in a human CRC cell line.
  • Fig. S5. Acute pathway modulation in PDX tumors.
  • Legend for table S1
  • Table S2. Top canonical pathways enriched among dynamic resistance genes.
  • Table S3. Top physiological processes enriched among dynamic resistance genes.

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of dynamic resistance genes identified by RNA-Seq.

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Citation: P. J. Belmont, P. Jiang, T. D. McKee, T. Xie, J. Isaacson, N. E. Baryla, J. Roper, M. J. Sinnamon, N. V. Lee, J. L. C. Kan, O. Guicherit, B. G. Wouters, C. A. O'Brien, D. Shields, P. Olson, T. VanArsdale, S. L. Weinrich, P. Rejto, J. G. Christensen, V. R. Fantin, K. E. Hung, E. S. Martin, Resistance to dual blockade of the kinases PI3K and mTOR in KRAS-mutant colorectal cancer models results in combined sensitivity to inhibition of the receptor tyrosine kinase EGFR. Sci. Signal. 7, ra107 (2014).

© 2014 American Association for the Advancement of Science