Supplementary Materials

Supplementary Materials for:

Large-scale functional analysis of the roles of phosphorylation in yeast metabolic pathways

Juliane Caroline Schulz, Mattia Zampieri, Stefanie Wanka, Christian von Mering, Uwe Sauer*

*Corresponding author. E-mail: sauer{at}imsb.biol.ethz.ch

This PDF file includes:

  • Fig. S1. Impact of kinase and phosphatase deletions on the metabolome.
  • Fig. S2. Number of enriched metabolic pathways per deletion strain.
  • Fig. S3. Growth profiles of yeast expressing mutant Lys20 or Lys21.
  • Fig. S4. Calculation of statistical threshold for metabolomic profiles.
  • Fig. S5. Schematic representation of the experimental setup and analysis workflow.

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Enzymes regulated by phosphorylation.
  • Table S2 (Microsoft Excel format). Details of MS ion annotation.
  • Table S3 (Microsoft Excel format). Fold change matrix of metabolite ion intensities.
  • Table S4 (Microsoft Excel format). Growth rate, metabolic impact, and metabolic similarity of kinase and phosphatase deletion strains.
  • Table S5 (Microsoft Excel format). GO enrichments for impact groups.
  • Table S6 (Microsoft Excel format). GO enrichments and STRING interactions for clusters.
  • Table S7 (Microsoft Excel format). KEGG pathway enrichment analysis of altered metabolites in kinase and phosphatase deletion strains.
  • Table S8 (Microsoft Excel format). Growth rates of ppq1Δ yeast grown on different carbon sources.
  • Table S9 (Microsoft Excel format). Phosphoproteomic changes in ppq1Δ yeast.
  • Table S10 (Microsoft Excel format). Predictions from locality scoring procedure.

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Citation: J. C. Schulz, M. Zampieri, S. Wanka, C. von Mering, U. Sauer, Large-scale functional analysis of the roles of phosphorylation in yeast metabolic pathways. Sci. Signal. 7, rs6 (2014).

© 2014 American Association for the Advancement of Science