Supplementary Materials

Supplementary Materials for:

E-cadherin interactome complexity and robustness resolved by quantitative proteomics

Zhenhuan Guo, Lisa J. Neilson, Hang Zhong, Paul S. Murray, Sara Zanivan, Ronen Zaidel-Bar*

*Corresponding author. E-mail: biezbr{at}nus.edu.sg

This PDF file includes:

  • Fig. S1. Relationship between the iBAQ sum and the number of lysines.
  • Fig. S2. E-cadherin–specific adhesive interaction between cells and E-cadherin–coated glass.
  • Fig. S3. Colocalization of GFP-fusion proteins with E-cadherin.
  • Fig. S4. Network topology of E-cad-BirA* interactome.
  • Fig. S5. E-cad-BirA* interactome graphed according to protein functional annotation.
  • Fig. S6. EGTA-induced loss of cell adhesion and recovery.
  • Fig. S7. Cortical localization of cadhesome proteins in isolated cells.

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). MS results of E-cadherin–proximal proteins from MKN28E-cad-BirA* cells.
  • Table S2 (Microsoft Excel format). MS results of E-cadherin adhesion plaques from cells adhering to E-cadherin–coated glass.
  • Table S3 (Microsoft Excel format). Localization of GFP-tagged proteins.
  • Table S4 (Microsoft Excel format). Protein interactions within E-cad-BirA* interactome predicted by PrePPI.
  • Table S5 (Microsoft Excel format). Protein domain analysis of the E-cad-BirA* interactome.

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Citation: Z. Guo, L. J. Neilson, H. Zhong, P. S. Murray, S. Zanivan, R. Zaidel-Bar, E-cadherin interactome complexity and robustness resolved by quantitative proteomics. Sci. Signal. 7, rs7 (2014).

© 2014 American Association for the Advancement of Science