Supplementary Materials

Supplementary Materials for:

The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling

Yulu C. Liu, Amber L. Couzens, Ashish R. Deshwar, Linda D. B. McBroom-Cerajewski, Xiaoyun Zhang, Vijitha Puviindran, Ian C. Scott, Anne-Claude Gingras, Chi-chung Hui, Stephane Angers*

*Corresponding author. E-mail: stephane.angers{at}utoronto.ca

This PDF file includes:

  • Fig. S1. Rescue of Kif7 expression in Kif7–/– MEFs.
  • Fig. S2. PPFIA1 and Kif7 interact through their coiled-coil domains.
  • Fig. S3. PPFIA1 is required for robust Gli1 induction during Hh pathway activation.
  • Fig. S4. PPFIA1 shRNA rescue experiments.
  • Fig. S5. Knockdown of Kif7 or PPFIA1 or use of a PP1A/PP2A inhibitor does not interfere with the ciliary localization of Smo during pathway activation.
  • Fig. S6. Phosphorylation of Kif7 affects its ciliary localization and Hh signaling.
  • Table S1. High-confidence data set reported in this study.
  • Table S2. Kif7 peptides quantified by MRMHR.
  • Table S3. List of primers.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 835 KB


Citation: Y. C. Liu, A. L. Couzens, A. R. Deshwar, L. D. B. McBroom-Cerajewski, X. Zhang, V. Puviindran, I. C. Scott, A.-C. Gingras, C.-c. Hui, S. Angers, The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling. Sci. Signal. 7, ra117 (2014).

© 2014 American Association for the Advancement of Science