Supplementary Materials

Supplementary Materials for:

The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192

Stine Granum,* Vibeke Sundvold-Gjerstad, Ramakrishna Prabhu Gopalakrishnan, Tone Berge, Lise Koll, Greger Abrahamsen, Morten Sørlie, Anne Spurkland*

*Corresponding author. E-mail: stine.granum{at}medisin.uio.no (S.G.); anne.spurkland{at}medisin.uio.no (A.S.)

This PDF file includes:

  • Fig. S1. Identification by fluorescence imagery of conjugates between Raji cells and transfected JCAM 1.6 cells.
  • Fig. S2. Distribution of Lck-SH2 domain binding preference to given phosphotyrosine motifs.
  • Table S1. List of Lck-interacting proteins.
  • Table S2. Summary per protein of peptide array screening of the binding of Lck-SH2 and Lck-SH2-Y192E to 431 phosphopeptides from Lck-interacting proteins.

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Citation: S. Granum, V. Sundvold-Gjerstad, R. P. Gopalakrishnan, T. Berge, L. Koll, G. Abrahamsen, M. Sørlie, A. Spurkland, The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192. Sci. Signal. 7, ra118 (2014).

© 2014 American Association for the Advancement of Science