Supplementary Materials

Supplementary Materials for:

Localized TRPA1 channel Ca2+ signals stimulated by reactive oxygen species promote cerebral artery dilation

Michelle N. Sullivan, Albert L. Gonzales, Paulo W. Pires, Allison Bruhl, M. Dennis Leo, Wencheng Li, Agathe Oulidi, Frederick A. Boop, Yumei Feng, Jonathan H. Jaggar, Donald G. Welsh, Scott Earley*

*Corresponding author. E-mail: searley{at}medicine.nevada.edu

This PDF file includes:

  • Fig. S1. NOX antibody specificity.
  • Fig. S2. NADPH oxidase proteins are present in myoendothelial projections.
  • Fig. S3. TRPA1 and NOX2 colocalize near IEL fenestrations.
  • Fig. S4. TRPA1 does not physically interact with NOX2 but is modified by 4-HNE.
  • Fig. S5. AITC stimulates Ca2+ influx in endothelial cells.
  • Fig. S6. TRPA1 sparklets in TRPA1-GFP–transfected HEK 293 cells.
  • Fig. S7. TRPA1 protein in mouse arteries.
  • Table S1. TRPA1 sparklet properties.
  • Table S2. Vascular reactivity of cerebral arteries from control and eTRPA1−/− mice.
  • Legend for movie S1

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.37 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mpg format). TRPA1 sparklets in endothelial cells.

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Citation: M. N. Sullivan, A. L. Gonzales, P. W. Pires, A. Bruhl, M. D. Leo, W. Li, A. Oulidi, F. A. Boop, Y. Feng, J. H. Jaggar, D. G. Welsh, S. Earley, Localized TRPA1 channel Ca2+ signals stimulated by reactive oxygen species promote cerebral artery dilation. Sci. Signal. 8, ra2 (2015).

© 2014 American Association for the Advancement of Science