Supplementary Materials

Supplementary Materials for:

STIM2 enhances receptor-stimulated Ca2+ signaling by promoting recruitment of STIM1 to the endoplasmic reticulum–plasma membrane junctions

Hwei Ling Ong, Lorena Brito de Souza, Changyu Zheng, Kwong Tai Cheng, Xibao Liu, Corinne M. Goldsmith, Stefan Feske, Indu S. Ambudkar*

*Corresponding author. E-mail: indu.ambudkar{at}nih.gov

This PDF file includes:

  • Fig. S1. Effects and specificity of targeted knockout of STIM2 in mouse salivary glands.
  • Fig. S2. Specificity of siRNA-mediated STIM2 knockdown and its effects on CCh-stimulated Ca2+ signaling.
  • Fig. S3. Effect of STIM1 or STIM2 knockdown on CCh-stimulated [Ca2+]i responses.
  • Fig. S4. Store depletion–induced clustering of STIM proteins in cells coexpressing YFP-STIM1 and CFP-STIM2 or expressing only YFP-STIM1EF→STIM2.
  • Fig. S5. Effect of deleting the polybasic tail domain of STIM1 on store depletion–induced clustering.

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Citation: H. L. Ong, L. B. de Souza, C. Zheng, K. T. Cheng, X. Liu, C. M. Goldsmith, S. Feske, I. S. Ambudkar, STIM2 enhances receptor-stimulated Ca2+ signaling by promoting recruitment of STIM1 to the endoplasmic reticulum–plasma membrane junctions. Sci. Signal. 8, ra3 (2015).

© 2014 American Association for the Advancement of Science