Supplementary Materials

Supplementary Materials for:

MAPK feedback encodes a switch and timer for tunable stress adaptation in yeast

Justin G. English, James P. Shellhammer, Michael Malahe, Patrick C. McCarter, Timothy C. Elston, Henrik G. Dohlman*

*Corresponding author. E-mail: hdohlman{at}

This PDF file includes:

  • Fig. S1. The antibodies generated against phosphorylated p38 recognize monophosphorylated and dually phosphorylated Hog1.
  • Fig. S2. Negative feedback only model fails to capture the Hog1 signaling profile.
  • Fig. S3. Hog1 signaling profile in the Ste505A strain.
  • Fig. S4. Hog1 signaling profile in ssk1Δ and rga1Δ yeast.
  • Fig. S5. Gating parameters for flow cytometry data.
  • Table S1. Transcription factor binding and interaction analysis.
  • Table S2. List of yeast strains used in this study.
  • Table S3. List of parameter values used in modeling.
  • Legends for database S1 and S2

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Technical Details

Format: Adobe Acrobat PDF

Size: 537 KB

Other Supplementary Material for this manuscript includes the following:

  • Database S1 (Microsoft Excel format). Annotated microarray data with GO analysis and organization.
  • Database S2 (Microsoft Excel format). Induction of 95 proteins from clusters 1 to 4 in response to 0, 350, or 650 mM KCl.

[Download databases S1 and S2]

Citation: J. G. English, J. P. Shellhammer, M. Malahe, P. C. McCarter, T. C. Elston, H. G. Dohlman, MAPK feedback encodes a switch and timer for tunable stress adaptation in yeast. Sci. Signal. 8, ra5 (2015).

© 2014 American Association for the Advancement of Science