Supplementary Materials

Supplementary Materials for:

Activation of TRPV1 channels inhibits mechanosensitive Piezo channel activity by depleting membrane phosphoinositides

Istvan Borbiro, Doreen Badheka, Tibor Rohacs*

*Corresponding author. E-mail: tibor.rohacs{at}rutgers.edu

This PDF file includes:

  • Fig. S1. Capsaicin-induced inward (TRPV1) current amplitudes in HEK293 cells transfected with Piezo1 and TRPV1.
  • Fig. S2. Effects of TRPV1 activation by low pH and TRPA1 activation by AITC on Piezo1 activity.
  • Fig. S3. Effects of PKC activation on Piezo1-mediated MA currents.
  • Fig. S4. Fluorescence-based PI(4,5)P2 and PI(4)P measurements in HEK293 cells cotransfected with hM1 or TRPV1 and various phosphoinositide sensors.
  • Fig. S5. Attempts to reactivate Piezo1 currents in excised inside-out patches in HEK293 cells.
  • Fig. S6. Cell-attached and excised inside-out patch clamp measurements of MA currents in Xenopus oocytes.

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Citation: I. Borbiro, D. Badheka, T. Rohacs, Activation of TRPV1 channels inhibits mechanosensitive Piezo channel activity by depleting membrane phosphoinositides. Sci. Signal. 8, ra15 (2015).

© 2015 American Association for the Advancement of Science