Supplementary Materials

Supplementary Materials for:

Single-cell analysis shows that paracrine signaling by first responder cells shapes the interferon-β response to viral infection

Sonali Patil, Miguel Fribourg, Yongchao Ge, Mona Batish, Sanjay Tyagi, Fernand Hayot, Stuart C. Sealfon*

*Corresponding author. E-mail: stuart.sealfon{at}mssm.edu

This PDF file includes:

  • Computational methods
  • Fig. S1. Validation of the single-molecule imaging of mRNA in situ.
  • Fig. S2.Temporal dynamics of single-cell Ifnb1 mRNA responses to viral infection at an MOI of 4.
  • Fig. S3. The percentages of Ifnb1-expressing cells are independent of the concentration of LPS.
  • Fig. S4. Temporal dispersion is a characteristic stochastic feature of the Ifnb1 response after viral infection.
  • Fig. S5. The faster dynamics of Ifnb1 expression in response to LPS does not change its characteristic propagation mode.
  • Fig. S6. Comparison between experimentally measured and simulated average numbers of Ifnb1 mRNAs per cell and the percentages of responding cells.
  • Fig. S7. Temporal dynamics of single-cell Ifnb1 responses in response to viral infection of A549 cells.
  • Fig. S8. Brefeldin A has no effect on the infectivity of A549 cells.
  • Table. S1. Sequences of oligonucleotide FISH probes specific for viral HN mRNA and tagged with Quasar 670.
  • Table. S2. Sequences of oligonucleotide FISH probes specific for Ifnb1 mRNA and tagged with Quasar 570.
  • Table. S3. Summary model parameter values.

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Citation: S. Patil, M. Fribourg, Y. Ge, M. Batish, S. Tyagi, F. Hayot, S. C. Sealfon, Single-cell analysis shows that paracrine signaling by first responder cells shapes the interferon-β response to viral infection. Sci. Signal. 8, ra16 (2015).

© 2015 American Association for the Advancement of Science