Supplementary Materials

Supplementary Materials for:

A molecular signature in the pannexin1 intracellular loop confers channel activation by the α1 adrenoreceptor in smooth muscle cells

Marie Billaud, Yu-Hsin Chiu, Alexander W. Lohman, Thibaud Parpaite, Joshua T. Butcher, Stephanie M. Mutchler, Leon J. DeLalio, Mykhaylo V. Artamonov, Joanna K. Sandilos, Angela K. Best, Avril V. Somlyo, Roger J. Thompson, Thu H. Le, Kodi S. Ravichandran, Douglas A. Bayliss, Brant E. Isakson*

*Corresponding author. E-mail: brant{at}virginia.edu

This PDF file includes:

  • Fig. S1. Effect of SMC deletion of Panx1 on Panx2 and Panx3 abundance, ATP content, α1DAR abundance, and phenylephrine-induced contraction of aortic rings.
  • Fig. S2. Effect of IL2 scrambled peptide and TAT peptide on phenylephrine-mediated constriction and ATP release.
  • Fig. S3. Effect of mutations in the Panx1 IL2 region on the channel function.
  • Table S1. Contractile properties of aortic rings isolated from control Cre+/Panx1Fl mice and Cre+/Panx1Fl injected with tamoxifen for 10 days.
  • Table S2. Effect of scrambled IL2 peptide and TAT peptide on constriction of TDAs in response to phenylephrine.
  • Table S3. Primers used for the generation of Panx1KYP>AAA, Panx1IVEQ>AAAA, and Panx1YLK>AAA plasmids.

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Citation: M. Billaud, Y.-H. Chiu, A. W. Lohman, T. Parpaite, J. T. Butcher, S. M. Mutchler, L. J. DeLalio, M. V. Artamonov, J. K. Sandilos, A. K. Best, A. V. Somlyo, R. J. Thompson, T. H. Le, K. S. Ravichandran, D. A. Bayliss, B. E. Isakson, A molecular signature in the pannexin1 intracellular loop confers channel activation by the α1 adrenoreceptor in smooth muscle cells. Sci. Signal. 8, ra17 (2015).

© 2015 American Association for the Advancement of Science