Supplementary Materials

Supplementary Materials for:

Viral entry route determines how human plasmacytoid dendritic cells produce type I interferons

Daniela Bruni, Maxime Chazal, Laura Sinigaglia, Lise Chauveau, Olivier Schwartz, Philippe Desprès, Nolwenn Jouvenet*

*Corresponding author. E-mail: jouvenet{at}pasteur.fr

This PDF file includes:

  • Fig. S1. Analysis of the depletion or isolation of pDCs from human PBMCs.
  • Fig. S2. TLR7 agonist–induced type I IFN production by pDCs is efficiently blocked by the specific TLR7 inhibitor IRS661.
  • Fig. S3. YF-17D replicates in Gen2.2 cells and stimulates the RIG-I signaling pathway.
  • Fig. S4. YF-17D induces nuclear translocation of IRF3 but not IRF7 in Gen2.2 cells.
  • Fig. S5. SeV stimulates RIG-I signaling in Gen2.2 cells.
  • Fig. S6. YF-17D–infected Huh7 cells stimulate IFNA expression in Gen2.2 cells.
  • Fig. S7. Sensing of YF-17D–infected cells stimulates nuclear translocation of IRF7 but not IRF3 in Gen2.2 cells.
  • Fig. S8. Infection with YF-17D stimulates nuclear translocation of IRF3 in Vero cells.
  • Fig. S9. Schematic representation of the proposed YF-17D–mediated signaling pathways in human pDCs.
  • Table S1. Primers used for semiquantitative and real-time PCR analysis.
  • Table S2. Reagents.
  • Table S3. Antibodies.
  • Table S4. Viruses.
  • Table S5. Primary cells and cell lines.
  • Table S6. shRNA sequences for knockdown experiments.

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Citation: D. Bruni, M. Chazal, L. Sinigaglia, L. Chauveau, O. Schwartz, P. Desprès, N. Jouvenet, Recruitment of the adaptor protein Nck to PECAM-1 couples oxidative stress to canonical NF-κB signaling and inflammation. Sci. Signal. 8, ra25 (2015).

© 2015 American Association for the Advancement of Science