Supplementary Materials

Supplementary Materials for:

Conformational activation of visual rhodopsin in native disc membranes

Erik Malmerberg, Petra H. M. Bovee-Geurts, Gergely Katona, Xavier Deupi, David Arnlund, Cecilia Wickstrand, Linda C. Johansson, Sebastian Westenhoff, Elena Nazarenko, Gebhard F. X. Schertler, Andreas Menzel, Willem J. de Grip, Richard Neutze*

*Corresponding author. E-mail: richard.neutze{at}chem.gu.se

This PDF file includes:

  • Fig. S1. Optical absorption spectra of rhodopsin disc membrane.
  • Fig. S2. Light-induced thermal signal within the experimental TR-WAXS data.
  • Fig. S3. SVD analysis of the TR-WAXS experimental data.
  • Fig. S4. Comparison of the difference WAXS spectra of the rhodopsin principal component and bacteriorhodopsin.
  • Fig. S5. Difference WAXS spectra predicted from deposited crystallographic structures.
  • Fig. S6. Comparison of the predicted WAXS curves with the experimental data during structural refinement.
  • Fig. S7. Predicted difference WAXS spectra when the optimal structural models were perturbed.
  • Table S1. Referenced crystallographic structures.

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Technical Details

Format: Adobe Acrobat PDF

Size: 2.11 MB

Other Supplementary Material for this manuscript includes the following:

  • PDB_S1. WAXS resting state monomer
  • PDB_S2. WAXS resting state dimer
  • PDB_S3. WAXS metaII state monomer
  • PDB_S4. WAXS metaII state dimer

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Citation: E. Malmerberg, P. H. M. Bovee-Geurts, G. Katona, X. Deupi, D. Arnlund, C. Wickstrand, L. C. Johansson, S. Westenhoff, E. Nazarenko, G. F. X. Schertler, A. Menzel, W. J. de Grip, R. Neutze, Conformational activation of visual rhodopsin in native disc membranes. Sci. Signal. 8, ra26 (2015).

© 2015 American Association for the Advancement of Science