Supplementary Materials

Supplementary Materials for:

PTEN inhibits PREX2-catalyzed activation of RAC1 to restrain tumor cell invasion

Sarah M. Mense, Douglas Barrows, Cindy Hodakoski, Nicole Steinbach, David Schoenfeld, William Su, Benjamin D. Hopkins, Tao Su, Barry Fine, Hanina Hibshoosh, Ramon Parsons*

*Corresponding author. E-mail: ramon.parsons{at}mssm.edu

This PDF file includes:

  • Fig. S1. PTEN loss is associated with poor prognosis in breast cancer; PREX2 is expressed in breast cancer.
  • Fig. S2. PTEN C2-tail suppresses invasion in BT549 and SUM149 cells; C2-tail does not antagonize PREX1-driven invasion.
  • Fig. S3. PTEN C2-tail does not alter PREX2 localization.
  • Fig. S4. PTEN inhibits PREX2-catalyzed RAC activation.
  • Fig. S5. PREX2 abundance in melanoma and pancreatic cancer cells and expression of PREX2 cancer mutants used in invasion studies.
  • Fig. S6. Purification of PREX2 mutants for use in nucleotide exchange assays.

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Citation: S. M. Mense, D. Barrows, C. Hodakoski, N. Steinbach, D. Schoenfeld, W. Su, B. D. Hopkins, T. Su, B. Fine, H. Hibshoosh, R. Parsons, PTEN inhibits PREX2-catalyzed activation of RAC1 to restrain tumor cell invasion. Sci. Signal. 8, ra32 (2015).

© 2015 American Association for the Advancement of Science