Supplementary Materials

Supplementary Materials for:

Molecular recognition of ketamine by a subset of olfactory G protein– coupled receptors

Jianghai Ho, Jose Manuel Perez-Aguilar, Lu Gao, Jeffery G. Saven, Hiroaki Matsunami, Roderic G. Eckenhoff*

*Corresponding author. E-mail: Roderic.Eckenhoff{at}uphs.upenn.edu

This PDF file includes:

  • Fig. S1. Alignment of the sequences of MOR136-1 and multiple templates.
  • Fig. S2. Comparison between the inactive- and active-based structures of MOR136-1.
  • Fig. S3. Additional docking calculation results that involve MOR136-1.
  • Fig. S4. Sequence alignment of receptors in the MOR136 family.
  • Fig. S5. Behavior of residues Asp1093.37 and Lys109 in the putative ketaminebinding pocket based on constrained MD simulations and molecular modeling.
  • Fig. S6. Analysis of the cell surface abundance of MOR136-1.
  • Fig. S7. Dose-response curves for the MOR136-1 F104Y mutant.
  • Fig. S8. Superimposed structures of MOR136-4 from MD simulations.
  • Fig. S9. Analysis of the cell surface abundance of MOR136-4.
  • Fig. S10. Analysis of the cell surface abundance of MOR136-11.
  • Fig. S11. Comparison between the MOR136-1–ketamine interaction and those of other ligand-GPCR complexes found in the CNS.
  • Table S1. Mouse ORs contained in the primary screen.
  • Table S2. Mouse ORs in the secondary screen.
  • Table S3. Solvent-accessible surface areas and volumes of the putative binding pockets of wild-type receptors and their mutants.
  • Table S4. Sequences of primers used to generate site-directed mutants of ORs.

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Citation: J. Ho, J. M. Perez-Aguilar, L. Gao, J. G. Saven, H. Matsunami, R. G. Eckenhoff, Molecular recognition of ketamine by a subset of olfactory G protein–coupled receptors. Sci. Signal. 8, ra33 (2015).

© 2015 American Association for the Advancement of Science