Supplementary Materials

Supplementary Materials for:

The mTORC1/4E-BP pathway coordinates hemoglobin production with ʟ-leucine availability

Jacky Chung, Daniel E. Bauer, Alireza Ghamari, Christopher P. Nizzi, Kathryn M. Deck, Paul D. Kingsley, Yvette Y. Yien, Nicholas C. Huston, Caiyong Chen, Iman J. Schultz, Arthur J. Dalton, Johannes G. Wittig, James Palis, Stuart H. Orkin, Harvey F. Lodish, Richard S. Eisenstein, Alan B. Cantor, Barry H. Paw*

*Corresponding author. E-mail: bpaw{at}rics.bwh.harvard.edu

This PDF file includes:

  • Fig. S1. Lat3 expression and function during erythroid development.
  • Fig. S2. LAT3 inhibition specifically decreases hemoglobinization.
  • Fig. S3. NEAA insufficiency does not affect hemoglobinization through mitochondrial function or iron uptake.
  • Fig. S4. mTORC1 activity is not affected by LAT3 inhibition in undifferentiated erythroid cells.
  • Fig. S5. 4E-BP proteins are critical regulators of hemoglobin translation.
  • Table S1. Essential amino acid composition.

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Citation: J. Chung, D. E. Bauer, A. Ghamari, C. P. Nizzi, K. M. Deck, P. D. Kingsley, Y. Y. Yien, N. C. Huston, C. Chen, I. J. Schultz, A. J. Dalton, J. G. Wittig, J. Palis, S. H. Orkin, H. F. Lodish, R. S. Eisenstein, A. B. Cantor, B. H. Paw, The mTORC1/4EBP pathway coordinates hemoglobin production with ʟ-leucine availability. Sci. Signal. 8, ra34 (2015).

© 2015 American Association for the Advancement of Science