Supplementary Materials for:

Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser¹⁶ to trigger its nuclear localization

Lina Herhaus, Ana B. Perez-Oliva, Giorgio Cozza, Robert Gourlay, Simone Weidlich, David G. Campbell, Lorenzo A. Pinna, Gopal P. Sapkota*

*Corresponding author. E-mail: g.sapkota{at}dundee.ac.uk

This PDF file includes:

• Fig. S1. ALK5 binds OTUB1 but does not induce OTUB1 Ser¹⁶ phosphorylation in cells.
• Fig. S2. CK2 substrates and pSer¹⁶-OTUB1 phosphorylation.
• Fig. S3. Interaction between OTUB1 and polyubiquitin chains.
• Fig. S4. Quantification of Fig. 5B.
• Fig. S5. Effects of leptomycin B and importazole on OTUB1 localization.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 912 KB

---

Citation: L. Herhaus, A. B. Perez-Oliva, G. Cozza, R. Gourlay, S. Weidlich, D. G. Campbell, L. A. Pinna, G. P. Sapkota, Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser¹⁶ to trigger its nuclear localization. Sci. Signal. 8, ra35 (2015).

© 2015 American Association for the Advancement of Science