Supplementary Materials

Supplementary Materials for:

Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization

Lina Herhaus, Ana B. Perez-Oliva, Giorgio Cozza, Robert Gourlay, Simone Weidlich, David G. Campbell, Lorenzo A. Pinna, Gopal P. Sapkota*

*Corresponding author. E-mail: g.sapkota{at}dundee.ac.uk

This PDF file includes:

  • Fig. S1. ALK5 binds OTUB1 but does not induce OTUB1 Ser16 phosphorylation in cells.
  • Fig. S2. CK2 substrates and pSer16-OTUB1 phosphorylation.
  • Fig. S3. Interaction between OTUB1 and polyubiquitin chains.
  • Fig. S4. Quantification of Fig. 5B.
  • Fig. S5. Effects of leptomycin B and importazole on OTUB1 localization.

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Citation: L. Herhaus, A. B. Perez-Oliva, G. Cozza, R. Gourlay, S. Weidlich, D. G. Campbell, L. A. Pinna, G. P. Sapkota, Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Sci. Signal. 8, ra35 (2015).

© 2015 American Association for the Advancement of Science