Supplementary Materials

Supplementary Materials for:

Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition

Andrea Blanka, Juliane Düvel, Andreas Dötsch, Birgit Klinkert, Wolf-Rainer Abraham, Volkhard Kaever, Christiane Ritter, Franz Narberhaus, Susanne Häussler*

*Corresponding author. E-mail: susanne.haeussler{at}twincore.de

This PDF file includes:

  • Fig. S1. Analysis of AccB production with His6 translational fusion constructs.
  • Fig. S2. Prediction of the secondary structures of DNA oligonucleotides and CD spectroscopy.
  • Fig. S3. Analysis of cellular fatty acid composition.
  • Fig. S4. Colony morphologies of complemented strains.
  • Fig. S5. Intracellular c-di-GMP concentrations for Rev1.
  • Fig. S6. Fatty acid composition under different growth conditions.
  • Fig. S7. Colony morphologies on Columbia blood agar plates.
  • Table S1. Strains and plasmids.
  • Table S2. Primers.
  • Table S3. DNA oligonucleotides used for CD spectroscopy.

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Citation: A. Blanka, J. Düvel, A. Dötsch, B. Klinkert, W.-R. Abraham, V. Kaever, C. Ritter, F. Narberhaus, S. Häussler, Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition. Sci. Signal. 8, ra36 (2015).

© 2015 American Association for the Advancement of Science