Supplementary Materials

Supplementary Materials for:

Protein kinase G–regulated production of H2S governs oxygen sensing

Guoxiang Yuan, Chirag Vasavda, Ying-Jie Peng, Vladislav V. Makarenko, Gayatri Raghuraman, Jayasri Nanduri, Moataz M. Gadalla, Gregg L. Semenza, Ganesh K. Kumar, Solomon H. Snyder, Nanduri R. Prabhakar*

*Corresponding author. E-mail: nanduri{at}

This PDF file includes:

  • Fig. S1. Western blot analysis of HEK-293 cells exogenously expressing HO-2 and CSE.
  • Fig. S2. Calcium-dependent calmodulin activity is not required for inhibition of CSE by CO.
  • Fig. S3. Effect of siRNA targeting sGCα1 on hypoxia-induced changes in cGMP concentrations and H2S generation in HEK-293 cells.
  • Fig. S4. Effects of YC-1 with or without either PKG or sGC inhibitor on H2S generation and the sensory nerve activity of wild-type and CSE−/− mouse carotid bodies.

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Citation: G. Yuan, C. Vasavda, Y.-J. Peng, V. V. Makarenko, G. Raghuraman, J. Nanduri, M. M. Gadalla, G. L. Semenza, G. K. Kumar, S. H. Snyder, N. R. Prabhakar, Protein kinase G–regulated production of H2S governs oxygen sensing. Sci. Signal. 8, ra37 (2015).

© 2015 American Association for the Advancement of Science