Supplementary Materials

Supplementary Materials for:

Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation

Kristina B. Emdal, Anna-Kathrine Pedersen, Dorte B. Bekker-Jensen, Kalliopi P. Tsafou, Heiko Horn, Sven Lindner, Johannes H. Schulte, Angelika Eggert, Lars J. Jensen, Chiara Francavilla,* Jesper V. Olsen*

*Corresponding author. E-mail: chiara.francavilla{at}cpr.ku.dk (C.F.); jesper.olsen{at}cpr.ku.dk (J.V.O.)

This PDF file includes:

  • Fig. S1. Characterization of the Tet-inducible system for TrkA expression and experimental workflow.
  • Fig. S2. Quality control of interactome and phosphoproteome data.
  • Fig. S3. Quality control of proteome data.
  • Fig. S4. TrkA modifications and depletion of Cbl family of proteins.
  • Fig. S5. Sustained signaling and sequence motif analysis.
  • Fig. S6. Effects of inhibition of sustained signaling on neurite outgrowth.
  • Fig. S7. Increased neurite outgrowth in neuroblastoma cell lines upon Cbl-b depletion.
  • Fig. S8. Cbl-b functions are specific for neuroblastoma cells.
  • Legends for tables S1 to S4

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Summary of SH-SY5Y-TR-TrkA interactome data.
  • Table S2 (Microsoft Excel format). Summary of data from two biological replicates of TrkA-expressing SH-SY5Y cells stimulated with NGF for 10, 45, and 120 min.
  • Table S3 (Microsoft Excel format). Summary of SH-SY5Y-TR-TrkA proteome data.
  • Table S4 (Microsoft Excel format). Summary of PC12 proteome data.

[Download Tables S1 to S4]


Citation: K. B. Emdal, A.-K. Pedersen, D. B. Bekker-Jensen, K. P. Tsafou, H. Horn, S. Lindner, J. H. Schulte, A. Eggert, L. J. Jensen, C. Francavilla, J. V. Olsen, Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. Sci. Signal. 8, ra40 (2015).

© 2015 American Association for the Advancement of Science