Supplementary Materials

Supplementary Materials for:

A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum

Runchi Gao, Siwei Zhao, Xupin Jiang, Yaohui Sun, Sanjun Zhao, Jing Gao, Jane Borleis, Stacey Willard, Ming Tang, Huaqing Cai, Yoichiro Kamimura, Yuesheng Huang, Jianxin Jiang, Zunxi Huang, Alex Mogilner, Tingrui Pan, Peter N. Devreotes, Min Zhao*

*Corresponding author. E-mail: minzhao{at}ucdavis.edu

This PDF file includes:

  • Fig. S1. The collection of mutant strains with morphological defects used in our screen.
  • Fig. S2. High-throughput screening strategy to determine electrotaxis phenotypes.
  • Fig. S3. Design and fabrication of the barcoded microplates.
  • Fig. S4. Cells on barcoded microplates at different positions in an electrotaxis chamber showed consistent electrotaxis responses.
  • Fig. S5. Dictyostelium cells displayed consistent migration phenotypes on both barcoded microplates and tissue culture dishes.
  • Fig. S6. Recapitulation of the defective electrotaxis phenotype in the mutant strains by knockout cells.
  • Fig. S7. Electrotaxis of vegetative cells.
  • Fig. S8. Mutated strains of Dictyostelium cells displayed consistent migration phenotypes on barcoded microplates and tissue culture dishes.
  • Table S1. Defective strains identified from the screen.
  • Table S2. Hyperresponsive strains identified.
  • Table S3. Knockouts confirmed the genes that underlie the 12 defective strains.
  • Legends for movies S1 to S4

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Wild-type AX2 cell not in an electric field.
  • Movie S2 (.avi format). Wild-type AX2 cell in an electric field of 12 V/cm.
  • Movie S3 (.avi format). PiaA in an electric field of 12 V/cm.
  • Movie S4 (.avi format). Reexpression of piaA in piaA cells restored electrotaxis.

[Download Movies S1 to S4]


Citation: R. Gao, S. Zhao, X. Jiang, Y. Sun, S. Zhao, J. Gao, J. Borleis, S. Willard, M. Tang, H. Cai, Y. Kamimura, Y. Huang, J. Jiang, Z. Huang, A. Mogilner, T. Pan, P. N. Devreotes, M. Zhao, A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum. Sci. Signal. 8, ra50 (2015).

© 2015 American Association for the Advancement of Science