Supplementary Materials

Supplementary Materials for:

Toll-like receptor 4 senses oxidative stress mediated by the oxidation of phospholipids in extracellular vesicles

Mateja Manček-Keber,* Mojca Frank-Bertoncelj, Iva Hafner-Bratkovič, Anže Smole, Mateja Zorko, Nina Pirher, Silvia Hayer, Veronika Kralj-Iglič, Blaž Rozman, Nejc Ilc, Simon Horvat, Roman Jerala*

*Corresponding author. E-mail: roman.jerala{at}ki.si (R.J.); mateja.mancek{at}ki.si (M.M.-K.)

This PDF file includes:

  • Fig. S1. Determination of the numbers of native EVs, stressEVs, and synEVs.
  • Fig. S2. Inhibition of LPS signaling also inhibits the activity of EVs.
  • Fig. S3. StressEVs activate the TLR4–MD-2 complex.
  • Fig. S4. 15-LO–hydroperoxylated PE compounds stimulate cytokine production, and 15-LO stimulates NF-κB signaling in a TLR4- and MD-2–dependent manner.
  • Fig. S5. 15-LO–hydroperoxylated PE compounds stimulate NF-κB activity in a TLR2-dependent manner.
  • Fig. S6. Heat maps of the pairwise signal comparison (Pearson’s correlation) and the 50 genes whose expression was most affected by stressEVs.
  • Fig. S7. Schematic representation of different phospholipid oxidation compounds, molecular complexes, and equilibria between MD-2 and different molecular species.
  • Fig. S8. Phospholipids bind to MD-2 at a region that overlaps with its LPS-binding site.

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Citation: M. Manček-Keber, M. Frank-Bertoncelj, I. Hafner-Bratkovič, A. Smole, M. Zorko, N. Pirher, S. Hayer, V. Kralj-Iglič, B. Rozman, N. Ilc, S. Horvat, R. Jerala, Toll-like receptor 4 senses oxidative stress mediated by the oxidation of phospholipids in extracellular vesicles.. Sci. Signal. 8, ra60 (2015).

© 2015 American Association for the Advancement of Science