Supplementary Materials

Supplementary Materials for:

IRE1 prevents endoplasmic reticulum membrane permeabilization and cell death under pathological conditions

Kohsuke Kanekura, Xiucui Ma, John T. Murphy, Lihua J. Zhu, Abhinav Diwan, Fumihiko Urano*

*Corresponding author. E-mail: urano{at}dom.wustl.edu

This PDF file includes:

  • Fig. S1. Leakage of ER contents to the cytosol precedes the apoptotic cascade.
  • Fig. S2. Phosphorylation of Bnip3.
  • Fig. S3. Role of Bnip3 in the leakage of ER calcium to the cytosol.
  • Fig. S4. Subcellular localization of Bnip3.
  • Fig. S5. Gel filtration of GRP94.
  • Fig. S6. Confirmation of attenuation of IRE1 signaling by small-molecule inhibitors.
  • Fig. S7. Leakage of ER contents to the cytosol in Xbp1+/+ and Xbp1−/− cells.
  • Fig. S8. Time-course analysis of the phosphorylation of JNK and leakage of ER contents.
  • Fig. S9. Degradation mechanism of Bnip3.
  • Fig. S10. Abundance of Bnip3 and phosphorylation of JNK and LC3-II in wild-type cells and Bax/Bak DKO cells.
  • Fig. S11. Live-cell imaging of MERO-GFP–expressing IRE1 knockout cells under ER stress conditions.
  • Fig. S12. Time-lapse ratio imaging of ER membrane permeabilization in IRE1 knockout cells.
  • Fig. S13. Time-lapse ratio imaging of HEK293 cells expressing cytosolic roGFP under ER stress conditions.
  • Fig. S14. Fluorescent imaging of HEK293 cells expressing the mCherry-Chop reporter under ER stress conditions.
  • Fig. S15. Scheme of ER membrane permeabilization by ER stress.

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Citation: K. Kanekura, X. Ma, J. T. Murphy, L. J. Zhu, A. Diwan, F. Urano, IRE1 prevents endoplasmic reticulum membrane permeabilization and cell death under pathological conditions. Sci. Signal. 8, ra62 (2015).

© 2015 American Association for the Advancement of Science