Supplementary Materials

Supplementary Materials for:

Regulation of brain glutamate metabolism by nitric oxide and S-nitrosylation

Karthik Raju, Paschalis-Thomas Doulias, Perry Evans, Elizabeth N. Krizman, Joshua G. Jackson, Oksana Horyn, Yevgeny Daikhin, Ilana Nissim, Marc Yudkoff, Itzhak Nissim, Kim A. Sharp, Michael B. Robinson, Harry Ischiropoulos*

*Corresponding author. E-mail: ischirop{at}mail.med.upenn.edu

This PDF file includes:

  • Fig. S1. Reduction in the enrichment of S-nitrosylated proteins in eNOS−/− and nNOS−/− mice.
  • Fig. S2. Additional biological processes and cellular functions identified for S-nitrosylated proteins in the mouse brain.
  • Fig. S3. Identification of 15N isotopic label in glutamate-associated metabolites.
  • Fig. S4. Characterization of GLT1-independent uptake in synaptosomes and GLT1-dependent uptake in cells.
  • Fig. S5. S-nitrosylation augments electrostatic potential.
  • Table S1. S-nitrosocysteine sites identified in wild-type, eNOS−/−, and nNOS−/− mouse brain.

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Citation: K. Raju, P.-T. Doulias, P. Evans, E. N. Krizman, J. G. Jackson, O. Horyn, Y. Daikhin, I. Nissim, M. Yudkoff, I. Nissim, K. A. Sharp, M. B. Robinson, H. Ischiropoulos, Regulation of brain glutamate metabolism by nitric oxide and S-nitrosylation. Sci. Signal. 8, ra68 (2015).

© 2015 American Association for the Advancement of Science