Supplementary Materials

Supplementary Materials for:

Proteotoxic stress reprograms the chromatin landscape of SUMO modification

Anne Seifert, Pietà Schofield, Geoffrey J. Barton, Ronald T. Hay*

*Corresponding author. E-mail: r.t.hay{at}dundee.ac.uk

This PDF file includes:

  • Fig. S1. SUMO-2 ChIP-seq data sets.
  • Fig. S2. Correlation of SUMO-2 peaks with features and genes.
  • Fig. S3. Examples of SUMO-2 peaks associated with different genomic annotations.
  • Fig. S4. Alignment of HS-induced SUMO-2 peaks with the TSSs of protein-coding genes.
  • Fig. S5. Co-occurrence of HS-induced SUMO-2 peaks and binding sites of selected transcription factors and chromatin-binding factors.
  • Fig. S6. Upstream regulatory pathway analysis for SUMO-2 target genes and genes not bound by SUMO-2.
  • Fig. S7. Effect of siRNA-mediated depletion of UBC9 on gene expression.
  • Fig. S8. Kinetics of the HS-induced recruitment of PIAS1 and SUMO-2 to chromatin.
  • Fig. S9. The HS-induced recruitment of SUMO to chromatin is part of the proteotoxic stress response.
  • Fig. S10. Relationship between HS-induced SUMO occupancy and the recruitment of HSF1 and UHRF1 to chromatin.
  • Fig. S11.SUMO localizes to the insoluble, DNA-associated nuclear fraction when subjected to HS.
  • Table S1. List of antibodies used in this study.
  • Table S2. List of PCR primers used in this study.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 3.39 MB


Citation: A. Seifert, P. Schofield, G. J. Barton, R. T. Hay, Proteotoxic stress reprograms the chromatin landscape of SUMO modification. Sci. Signal. 8, rs7 (2015).

© 2015 American Association for the Advancement of Science