Supplementary Materials for:

The interaction of heparan sulfate proteoglycans with endothelial transglutaminase-2 limits VEGF₁₆₅-induced angiogenesis

Nathan Beckouche, Marine Bignon, Virginie Lelarge, Thomas Mathivet, Cathy Pichol-Thievend, Sarah Berndt, Julie Hardouin, Marion Garand, Corinne Ardidie-Robouant, Alain Barret, Gerry Melino, Hugues Lortat-Jacob, Laurent Muller, Catherine Monnot,* Stephane Germain

*Corresponding author. E-mail: catherine.monnot{at}college-de-france.fr

This PDF file includes:

• Fig. S1. Analysis of retinas and aortas from tg2^+/+ and tg2^−/− mice.
• Fig. S2. TG2 accumulation in ECM and TG2 silencing in endothelial cells.
• Fig. S3. Proliferation, β₁ integrin–mediated sprouting, and fibronectin abundance and deposition in TG2-silenced endothelial cells.
• Fig. S4. VEGFR2 internalization in response to VEGF₁₆₅.
• Fig. S5. TG2 silencing does not affect VEGF₁₂₁-induced signaling.
• Fig. S6. HS content and deposition.
• Fig. S7. Reexpression of wild-type and mutant TG2 in TG2-silenced endothelial cells.

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Citation: N. Beckouche, M. Bignon, V. Lelarge, T. Mathivet, C. Pichol-Thievend, S. Berndt, J. Hardouin, M. Garand, C. Ardidie-Robouant, A. Barret, G. Melino, H. Lortat-Jacob, L. Muller, C. Monnot, S. Germain, The interaction of heparan sulfate proteoglycans with endothelial transglutaminase-2 limits VEGF₁₆₅-induced angiogenesis. Sci. Signal. 8, ra70 (2015).

© 2015 American Association for the Advancement of Science