Supplementary Materials

Supplementary Materials for:

A dual role for NOTCH signaling in joint cartilage maintenance and osteoarthritis

Zhaoyang Liu, Jianquan Chen, Anthony J. Mirando, Cuicui Wang, Michael J. Zuscik, Regis J. O'Keefe, Matthew J. Hilton*

*Corresponding author. E-mail: matthew.hilton{at}dm.duke.edu

This PDF file includes:

  • Fig. S1. Development of a NOTCH1 activation model with the combined Tet-On/Cre system.
  • Fig. S2. Sustained activation of NOTCH1 signaling in postnatal chondrocytes results in increased chondrocyte apoptosis.
  • Fig. S3. Sustained activation of NOTCH1 signaling in postnatal chondrocytes results in a progressive, OA-like pathology.
  • Fig. S4. Transient activation of NOTCH1 signaling in postnatal chondrocytes results in increased synthesis of cartilage ECM and joint maintenance for as long as 3
    months after injection with DOX, but does not protect from cartilage degradation after MLI surgery.
  • Fig. S5. Suppression of tyrosine kinase signaling leads to selected effects on NOTCH-regulated genes.
  • Fig. S6. Suppression of GPCR signaling leads to selected effects on NOTCH-regulated genes.
  • Fig. S7. Suppression of NO signaling leads to selected effects on NOTCH-regulated genes.

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Citation: Z. Liu, J. Chen, A. J. Mirando, C. Wang, M. J. Zuscik, R. J. O'Keefe, M. J. Hilton, A dual role for NOTCH signaling in joint cartilage maintenance and osteoarthritis. Sci. Signal. 8, ra71 (2015).

© 2015 American Association for the Advancement of Science