Supplementary Materials

Supplementary Materials for:

Protein phosphatase 2A regulatory subunit B56α limits phosphatase activity in the heart

Sean C. Little, Jerry Curran, Michael A. Makara, Crystal F. Kline, Hsiang-Ting Ho, Zhaobin Xu, Xiangqiong Wu, Iuliia Polina, Hassan Musa, Allison M. Meadows, Cynthia A. Carnes, Brandon J. Biesiadecki, Jonathan P. Davis, Noah Weisleder, Sandor Györke, Xander H. Wehrens, Thomas J. Hund, Peter J. Mohler*

*Corresponding author. E-mail: peter.mohler{at}osumc.edu

This PDF file includes:

  • Fig. S1. B56α+/− mice display reduced B56α abundance without changes in the abundance of PP2A core subunits.
  • Fig. S2. Wild-type and B56α+/− mice have similar QT intervals.
  • Fig. S3. Wild-type and B56α+/− mice have similar peak heart rates after exercise.
  • Fig. S4. B56α+/− mice display an aberrant response to adrenergic stimulation.
  • Fig. S5. B56α−/− mice display phenotypes associated with increased parasympathetic activity.
  • Fig. S6. B56α+/− mice display exaggerated cholinergic response to carbachol administration.
  • Fig. S7. B56α+/− and B56α−/− mice display normal ECG responses after inhibition of muscarinic acetylcholine receptors.
  • Fig. S8. B56α-deficient mice display reduced heart rate after inhibition of sympathetic and parasympathetic signaling.
  • Fig. S9. B56α−/− hearts display reduced phosphorylation of RyR2.
  • Fig. S10. B56α associates with PP2A/C and RyR2 but is not required for the interaction between RyR2 and PP2A/C.
  • Fig. S11. B56α+/− and B56α−/− atria display reduced phosphorylation of RyR2.
  • Fig. S12. Myofilament proteins are phosphorylated to a similar extent in wild-type and B56α+/− mice.
  • Fig. S13. The PP2A core enzyme is differentially localized in B56α+/− and B56α−/− myocytes.
  • Fig. S14. B56α abundance is increased in ankyrin-B–deficient hearts.

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Citation: S. C. Little, J. Curran, M. A. Makara, C. F. Kline, H.-T. Ho, Z. Xu, X. Wu, I. Polina, H. Musa, A. M. Meadows, C. A. Carnes, B. J. Biesiadecki, J. P. Davis, N. Weisleder, S. Györke, X. H. Wehrens, T. J. Hund, P. J. Mohler, Protein phosphatase 2A regulatory subunit B56α limits phosphatase activity in the heart. Sci. Signal. 8, ra72 (2015).

© 2015 American Association for the Advancement of Science