Supplementary Materials

Supplementary Materials for:

Real-time dynamics of peptide ligand–dependent receptor complex formation in planta

Marc Somssich, Qijun Ma, Stefanie Weidtkamp-Peters, Yvonne Stahl, Suren Felekyan, Andrea Bleckmann, Claus A. M. Seidel,* Rüdiger Simon*

*Corresponding author. E-mail: ruediger.simon{at}hhu.de (R.S.); cseidel{at}hhu.de (C.A.M.S.)

This PDF file includes:

  • Fig. S1. Fluorescence intensity, lifetime, and anisotropy projections on a cell expressing BAK1-GFP and FLS2-mCherry that was exposed to the control peptide.
  • Fig. S2. Fluorescence intensity, lifetime, and anisotropy projections on a cell expressing BAK1-GFP and FLS2-mCherry that was exposed to flg22.
  • Fig. S3. Time series of FRET efficiencies (E) and fraction of FRET-active complexes (xFRET) of individual cells after peptide treatment.
  • Fig. S4. Fluorescence intensity, lifetime, and anisotropy projections on a cell expressing CRN-GFP, CLV2, and CLV1-mCherry that was exposed to the control peptide.
  • Fig. S5. Fluorescence intensity, lifetime, and anisotropy projections on a cell expressing CRN-GFP, CLV2, and CLV1-mCherry that was exposed to CLV3.

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Citation: M. Somssich, Q. Ma, S. Weidtkamp-Peters, Y. Stahl, S. Felekyan, A. Bleckmann, C. A. M. Seidel, R. Simon, Real-time dynamics of peptide ligand– dependent receptor complex formation in planta. Sci. Signal. 8, ra76 (2015).

© 2015 American Association for the Advancement of Science