Supplementary Materials

Supplementary Materials for:

The E3 ligase APC/CCdh1 promotes ubiquitylation-mediated proteolysis of PAX3 to suppress melanocyte proliferation and melanoma growth

Juxiang Cao, Xiangpeng Dai, Lixin Wan, Hongshen Wang, Jinfang Zhang, Philip S. Goff, Elena V. Sviderskaya, Zhenyu Xuan, Zhixiang Xu, Xiaowei Xu, Philip Hinds, Keith T. Flaherty, Douglas V. Faller, Colin R. Goding, Yongjun Wang,* Wenyi Wei,* Rutao Cui*

*Corresponding author. E-mail: yjwang88{at}hotmail.com (Y.W.); wwei{at}bidmc.harvard.edu (W.W.); rutaocui{at}bu.edu (R.C.)

This PDF file includes:

  • Fig. S1. Correlation and colocalization between Cdh1 and PAX3 abundance in normal skin and melanoma tissue.
  • Fig. S2. Depletion of endogenous Cdh1 does not affect PAX3 mRNA expression.
  • Fig. S3. Detection of colocalization between Cdh1 and PAX3 in melanocytes and melanoma cell lines.
  • Fig. S4. APC/CCdh1 E3 ligase does not target MITF in melanocytes.
  • Fig. S5. Interaction with PAX3 is impaired by the expression of melanoma-derived Cdh1 mutants.
  • Fig. S6. Depletion of endogenous Cdh1 in mouse and human melanocytes increases MITF and tyrosinase signaling.
  • Fig. S7. Cdh1 silencing decreases doxorubicin sensitivity in melanoma cells.
  • Table S1. Genetic mutations of APC/C components in TCGA melanomas and exome sequencing data.

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Citation: J. Cao, X. Dai, L. Wan, H. Wang, J. Zhang, P. S. Goff, E. V. Sviderskaya, Z. Xuan, Z. Xu, X. Xu, P. Hinds, K. T. Flaherty, D. V. Faller, C. R. Goding, Y. Wang, W. Wei, R. Cui, The E3 ligase APC/CCdh1 promotes ubiquitylation-mediated proteolysis of PAX3 to suppress melanocyte proliferation and melanoma growth. Sci. Signal. 8, ra87 (2015).

© 2015 American Association for the Advancement of Science