Supplementary Materials

Supplementary Materials for:

Inactivation of PYR/PYL/RCAR ABA receptors by tyrosine nitration may enable rapid inhibition of ABA signaling by nitric oxide in plants

Mari-Cruz Castillo, Jorge Lozano-Juste, Miguel González-Guzmán, Lesia Rodriguez, Pedro L. Rodriguez, José León*

*Corresponding author. E-mail: jleon{at}ibmcp.upv.es

This PDF file includes:

  • Fig. S1. Dose-dependent nitration of PYR/PYL/RCAR proteins by the peroxynitrite donor SIN-1.
  • Fig. S2. Effect of increasing concentrations of the nitrosylating reagent GSNO on PYR/PYL/RCAR function.
  • Fig. S3. Amino acid sequence alignment of PYR/PYL/RCAR from A. thaliana and other plants.
  • Fig. S4. Models of 3D structures of PYR1.
  • Fig. S5. Simultaneous production of NO and superoxide anion in roots treated with ABA.
  • Fig. S6. Schematic representation of the experimental system designed for in planta proteomic analyses.
  • Fig. S7. In silico analysis of solvent accessibility in PYR1.
  • Table S1. Spatial parameters of PYR1 Tyr residues.
  • Legends for tables S2 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Proteomic analyses of in vitro nitrated PYR1.
  • Table S3 (Microsoft Excel format). Peptides and PTMs identified in the in vivo proteomic analyses.
  • Table S4 (Microsoft Excel format). Peptides and PTMs identified from HA-tagged PYR1 proteins processed under reducing conditions.

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Citation: M.-C. Castillo, J. Lozano-Juste, M. González-Guzmán, L. Rodriguez, P. L. Rodriguez, J. León, Inactivation of PYR/PYL/RCAR ABA receptors by tyrosine nitration may enable rapid inhibition of ABA signaling by nitric oxide in plants. Sci. Signal. 8, ra89 (2015).

© 2015 American Association for the Advancement of Science