Supplementary Materials

Supplementary Materials for:

Systematic profiling of the bacterial phosphoproteome reveals bacterium-specific features of phosphorylation

Miao-Hsia Lin, Naoyuki Sugiyama, Yasushi Ishihama*

*Corresponding author. E-mail: yishiham{at}pharm.kyoto-u.ac.jp

This PDF file includes:

  • Fig. S1. TIC chromatograms of samples with different pretreatments.
  • Fig. S2. Coexistence of serine, threonine, and tyrosine phosphorylation with aspartate and histidine phosphorylation in the PTS and two-component systems.
  • Fig. S3. Patterns of position-directed phosphorylation from prokaryotes to mammals.
  • Fig. S4. Enrichment reproducibility in technical and biological replicates.
  • Fig. S5. Comparison of enrichment efficiency among three bacterial strains.

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Technical Details

Format: Adobe Acrobat PDF

Size: 510 KB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of identified phosphopeptides in E. coli.
  • Table S2 (Microsoft Excel format). List of identified phosphopeptides in B. subtilis.
  • Table S3 (Microsoft Excel format). List of identified phosphopeptides in K. pneumoniae.
  • Table S4 (Microsoft Excel format). Phosphorylation motifs found in bacteria.
  • Table S5 (Microsoft Excel format). Reproducibility of technical replicates.

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Citation: M.-H. Lin, N. Sugiyama, Y. Ishihama, Systematic profiling of the bacterial phosphoproteome reveals bacterium-specific features of phosphorylation. Sci. Signal. 8, rs10 (2015).

© 2015 American Association for the Advancement of Science