Supplementary Materials

Supplementary Materials for:

Frontrunners of T cell activation: Initial, localized Ca2+ signals mediated by NAADP and the type 1 ryanodine receptor

Insa M. A. Wolf, Björn-Philipp Diercks, Ellen Gattkowski, Frederik Czarniak, Jan Kempski, René Werner, Daniel Schetelig, Hans-Willi Mittrücker, Valéa Schumacher, Manuel von Osten, Dimitri Lodygin, Alexander Flügel, Ralf Fliegert, Andreas H. Guse*

*Corresponding author. E-mail: guse{at}uke.de

This PDF file includes:

  • Fig. S1. Properties of Ca2+ indicators.
  • Fig. S2. Comparison of F/F0 Fluo-4 ratioing to Fluo-4/Fura Red ratioing during localized T cell activation.
  • Fig. S3. Comparison of the Fluo-4/Fura Red ratio in Jurkat cells and primary mouse T cells as compared to that in a cell-free solution.
  • Fig. S4. Comparison of the intracellular distributions of the Ca2+ indicators Fluo-4 and Fura Red with that of Fura-2 in Jurkat cells.
  • Fig. S5. Effect of knockdown of RyRs on global Ca2+ signaling in Jurkat cells.
  • Fig. S6. Kinetics and amplitude of localized Ca2+ signals in individual primary mouse T cells.
  • Fig. S7. Short-lived, initial Ca2+ signals in a responding primary T cell from RyR1−/− mice.
  • Fig. S8. Effect of knockdown of RyRs on Ca2+ signaling evoked by microinjection of NAADP into Jurkat cells.
  • Fig. S9. Calculation of the Kd for Fluo-4/Fura Red in situ.

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Citation: I. M. A. Wolf, B.-P. Diercks, E. Gattkowski, F. Czarniak, J. Kempski, R. Werner, D. Schetelig, H.-W. Mittrücker, V. Schumacher, M. von Osten, D. Lodygin, A. Flügel, R. Fliegert, A. H. Guse, Frontrunners of T cell activation: Initial, localized Ca2+ signals mediated by NAADP and the type 1 ryanodine receptor. Sci. Signal. 8, ra102 (2015).

© 2015 American Association for the Advancement of Science