Supplementary Materials

Supplementary Materials for:

Quantitative phosphoproteomics reveals new roles for the protein phosphatase PP6 in mitotic cells

Scott F. Rusin, Kate A. Schlosser, Mark E. Adamo, Arminja N. Kettenbach*

*Corresponding author. E-mail: arminja.n.kettenbach{at}dartmouth.edu

This PDF file includes:

  • Fig. S1. Characterization of PP6c shRNAs.
  • Fig. S2. Quantitative comparison of protein abundance changes upon PP6c depletion with individual PP6c shRNAs.
  • Fig. S3. Scheme of cell cycle synchronization.
  • Fig. S4. Characterization of PP6c-regulated phosphopeptides.
  • Fig. S5. LC-MS/MS trace and MS/MS spectra of known PP6c substrate DNA-PK pSer3205.
  • Fig. S6. Interaction network of proteins with increased and decreased phosphorylation upon PP6c depletion.
  • Fig. S7. LC-MS/MS trace and MS/MS spectra of NCAP-G pSer973/5.
  • Fig. S8. Characterization of PP6c and NCAP-G purifications.
  • Fig. S9. NCAP-G analysis.
  • Legend for tables S1 to S8

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Table containing TMT protein quantification results.
  • Table S2 (Microsoft Excel format). Table containing significantly increased phosphopeptides due to phosphorylation or protein abundance, significantly decreased phosphopeptides due to phosphorylation or protein abundance, and all phosphopeptide and protein data.
  • Table S3 (Microsoft Excel format). Table containing quantification results of AURKA substrates.
  • Table S4 (Microsoft Excel format). Table containing quantification results of RXS motif–containing phosphopeptides that increase in phosphorylation occupancy upon PP6c depletion.
  • Table S5 (Microsoft Excel format). Table containing proteins identified in 3×Flag-NCAP-H and 3×Flag-PP6c purification.
  • Table S6 (Microsoft Excel format). Table containing quantification results of the PP6c in vitro phosphatase assays of NCAP-G.
  • Table S7 (Microsoft Excel format). Table containing quantification results of the CK2 in vitro kinase assay of NCAP-G.
  • Table S8 (Microsoft Excel format). Table containing quantification results of proteinase K digest of NCAP-G.

Citation: S. F. Rusin, K. A. Schlosser, M. E. Adamo, A. N. Kettenbach, Quantitative phosphoproteomics reveals new roles for the protein phosphatase PP6 in mitotic cells. Sci. Signal. 8, rs12 (2015).

© 2015 American Association for the Advancement of Science