Supplementary Materials

Supplementary Materials for:

CD4+ T cell–dependent and CD4+ T cell–independent cytokine-chemokine network changes in the immune responses of HIV-infected individuals

Kelly B. Arnold, Gregory L. Szeto, Galit Alter, Darrell J. Irvine,* Douglas A. Lauffenburger*

*Corresponding author. E-mail: djirvine{at}mit.edu (D.J.I.);lauffen{at}mit.edu (D.A.L.)

This PDF file includes:

  • Fig. S1. Analysis of cytokine production by PBMCs from healthy donors.
  • Fig. S2. Comparison of PLSDA classification with classification based on individual cytokines.
  • Fig. S3. Experimental validation of PLSDA results with PBMCs from healthy donors.
  • Fig. S4. Analysis of the cellular composition of PBMCs from various donors.
  • Fig. S5. Full, unpruned decision tree.
  • Fig. S6. Analysis of the percentages of IL-2–secreting cells in PBMCs stimulated with anti-CD3/CD28 beads.
  • Fig. S7. Analysis of the percentages of IFN-γ–secreting cells in PBMCs stimulated with R848 or LPS.
  • Fig. S8. Consistent, enhanced secretion of TNF-α by monocytes of all cohorts.
  • Fig. S9. Decision tree sensitivity analysis.
  • Fig. S10. Minimal standard curve variance between batches and patient cohorts.
  • Table S1. Matrix of statistical significance tests for all pairwise univariate comparisons.
  • Table S2. HIV-infected donor information.
  • Table S3. Correlations between cytokine secretion at 6 hours and the divergence in cytokine profiles 72 hours after innate immune stimulation.

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Citation: K. B. Arnold, G. L. Szeto, G. Alter, D. J. Irvine, D. A. Lauffenburger, CD4+ T cell– dependent and CD4+ T cell–independent cytokine-chemokine network changes in the immune responses of HIV-infected individuals. Sci. Signal. 8, ra104 (2015).

© 2015 American Association for the Advancement of Science