Supplementary Materials

Supplementary Materials for:

Abnormal mechanosensing and cofilin activation promotes the progression of ascending aortic aneurysms in mice

Yoshito Yamashiro, Christina L. Papke, Jungsil Kim, Lea-Jeanne Ringuette, Qing-Jun Zhang, Zhi-Ping Liu, Hamid Mirzaei, Jessica E. Wagenseil, Elaine C. Davis, Hiromi Yanagisawa*

*Corresponding author. E-mail: hkyanagisawa{at}tara.tsukuba.ac.jp

This PDF file includes:

  • Fig. S1. ACE abundance and ERK phosphorylation in Fbln4SMKO aortas after P7.
  • Fig. S2. Ontology analysis and proteins that showed changes in abundance during aneurysm development.
  • Fig. S3. qPCR analysis of genes encoding the proteins identified by 2D-DIGE.
  • Fig. S4. Phosphorylation of cofilin in Fbln4SMKO descending aortas.
  • Fig. S5. Activated RhoA signaling in Fbln4SMKO ascending aortas.
  • Fig. S6. Dephosphorylation of cofilin by SSH1 in rat vascular SMCs.
  • Fig. S7. Phosphorylation of FAK and ILK in ascending aortas.
  • Fig. S8. The effects of PI3K inhibitors on general growth and aneurysm development.
  • Fig. S9. The effect of LY294002 on aneurysm development in Fbln4SMKO mice.
  • Fig. S10. Evaluation of PKD phosphorylation and calcineurin activity in ascending aortas.
  • Table S1. Antibodies used in this study.
  • Table S2. qPCR primer sequences.
  • Table S3. List of links to raw data for mass spectrometry.

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Citation: Y. Yamashiro, C. L. Papke, J. Kim, L.-J. Ringuette, Q.-J. Zhang, Z.-P. Liu, H. Mirzaei, J. E. Wagenseil, E. C. Davis, H. Yanagisawa, Abnormal mechanosensing and cofilin activation promotes the progression of ascending aortic aneurysms in mice. Sci. Signal. 8, ra105 (2015).

© 2015 American Association for the Advancement of Science