Supplementary Materials

Supplementary Materials for:

Genome-wide RNAi screening implicates the E3 ubiquitin ligase Sherpa in mediating innate immune signaling by Toll in Drosophila adults

Hirotaka Kanoh, Li-Li Tong, Takayuki Kuraishi,* Yamato Suda, Yoshiki Momiuchi, Fumi Shishido, Shoichiro Kurata*

*Corresponding author. E-mail: akayuki.kuraishi{at}gmail.com (T.K.); kurata{at}mail.pharm.tohoku.ac.jp (S.K.)

This PDF file includes:

  • Fig. S1. Numbers of candidate genes for each screening sorted by functional annotations.
  • Fig. S2. Expression profiles of sherpa and the sherpa1 loss-of-function mutant.
  • Fig. S3. Knockdown efficiency of sherpa and smt3 in DL1 cells.
  • Fig. S4. Quantification of coimmunoprecipitated proteins and posttranslational modifications, including polyubiquitylation.
  • Fig. S5. Western blotting analysis of Tube coexpressed with dMyd88 and Smt3.
  • Fig. S6. Expression of Drosomycin and Diptericin mRNAs in Pitslre-RNAi flies and analysis of Drosomycin promoter activity in Doa-RNAi cells.
  • Table S1. Sequences of DNA primers used for dsRNA synthesis.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). List of candidate genes for each screening sorted by functional annotation.

Citation: H. Kanoh, L.-L. Tong, T. Kuraishi, Y. Suda, Y. Momiuchi, F. Shishido, S. Kurata, Genome-wide RNAi screening implicates the E3 ubiquitin ligase Sherpa in mediating innate immune signaling by Toll in Drosophila adults. Sci. Signal. 8, ra107 (2015).

© 2015 American Association for the Advancement of Science